Beta-lactams, Tetracyclines, Streptomycin, Chloramphenicol
and Cefalexin 5 in 1 Rapid Test Kit
If the milk containing antibiotics is taken for a long time, the sensitive bacteria in the body will be killed or suppressed, but the resistant bacteria will multiply, which will break the original balance and cause long-term diarrhea or malnutrition. In severe cases, it can also cause resistant bacteria infection. The detection of antibiotic residues in raw milk is of great concern for the dairy industry because of the potential interfering and adverse effects of antibiotic residues frequently found in raw milk.
Experimental Preparation:
Restore the sample and kit to room temperature.
Operation steps:
1. Open the reagent barrel and take out the required number of micropores. Put the rest back into the reagent barrel and cover it well to avoid the reagent in the reagent barrel getting damp.
2. Mix the sample evenly and suck 200 μ L in micropores, blow and mix evenly for 5 ~ 10 times, and incubate at 40 ℃ for 3min.
3. Take out the required number of test strips, cover the reagent barrel cover, and then mark one end of the absorbent paper of the test strip.
4. Insert one end of the sample pad of the test strip into the micropore, react at 40 ℃ for 5min, interpret the results according to the schematic diagram, and the interpretation at other times is invalid.
Precautions:
1. Do not mix different batches of test strips and micropores.
2. It is recommended to wear clean disposable gloves for operation.
3. Retest the positive test results.
4. The reagent barrel shall be used up within 1 week after opening, and the unused reagent shall be stored at 2 ~ 8 ℃.
5. This product is only used for preliminary screening.
| Analyte | Beta-lactams, Tetracyclines, Streptomycin, Chloramphenicol and Cefalexin |
| Sample Type | Raw cow's milk |
| Methodology | Colloidal Gold Immunochromatography Lateral Flow Assay |
| Packaging | 96 Tests |
| Storage | The kits should be stored at a dry place away from the sunlight and the storage temperature is 2-8˚C. DO NOT FREEZE! |
| Shelf life | The validity for this kit is 12 months |
| Application | This test is designed for on-site rapid detection or daily control in laboratories. |
Limit of Detection (μg/kg=ppb):
| Tetracyclines |
|
|
| Antibiotic | EU MRL (μg/kg) | LOD (μg/kg) |
| Tetracycline | 100 | 7-10 |
| Doxycycline | * | 7-10 |
| Oxytetracycline | 100 | 7-10 |
| Chlortetracycline | 100 | 7-10 |
| Chloramphenicol |
|
|
| Antibiotic | EU MRL (μg/kg) | LOD (μg/kg) |
| Chloramphenicol | ** | 0.3 |
| Streptomycin/Dihydrostreptomycin |
|
|
| Antibiotic | EU MRL (μg/kg) | LOD (μg/kg) |
| Streptomycin | 200 | 50 |
| Dihydrostreptomycin | 200 | 50 |
| Penicillins |
|
|
| Antibiotic | EU MRL (μg/kg) | LOD (μg/kg) |
| Penicillin G | 4 | 1-2 |
| Cloxacillin | 30 | 3-6 |
| Amoxicillin | 4 | 2-3 |
| Oxacillin | 30 | 5-7 |
| Nafcillin | 30 | 7-10 |
| Ampicillin | 4 | 2-3 |
| Dicloxacillin | 30 | 3-6 |
| Benzathine | / | 3-5 |
| Cephalosporin |
|
|
| Antibiotic | EU MRL (μg/kg) | LOD (μg/kg) |
| Cefquinome | 20 | 5-7 |
| Cefalonium | 20 | 3-5 |
| Ceftiofur | 100 | 70-90 |
| Cefapirin | 60 | 4-8 |
| Cefoperazone | 50 | 3-5 |
| Cefacetrile | 125 | 15-20 |
| Cefazolin | 50 | 20-30 |
| Cefalexin | 100 | 10-15 |
Provided Materials and Reagent：
1.Test strips: 12x8 strips (96 strips total)
2.Microwells: 12x8 wells (96 wells total)
3.Positive control: 1 (Tetracycline: 10ppb, Chloramphenicol:0.3ppb, Streptomycin: 50ppb, Penicillin G: 2ppb, Cefalexin: 15ppb)
4.Negative control: 1
5.Pipette tips: 100/kit
6.Pipette:1
7.Microwell holder: 1
Materials Required But Not Provided:
1.Micropipette
2.Timer
3.Incubator
4.Reader (optional)
