Eclipse 3G test kit – 96 tests
ECLIPSE is a test based on the inhibition of microbial growth. It is supplied in a microtiter plate format where each well contains agar medium spread with Geobacillus stearothermophilus spores and a pH indicator.
When the plate is incubated at 65ºC, spores germinate and cells grow producing acid and changing the agar pH. Variations of pH will produce changes of the agar colour from blue (purple) to yellowish. When milk samples contain inhibitors at higher concentrations than the detection limit, microorganisms will not grow and neither colour changes will be observed.
ECLIPSE 3G tests are used with dry incubator Milkotester ET- W24. ECLIPSE 3G detects inhibitors from most used groups of antibiotics in animal husbandry – β-lactam, tetracycline, sulfamides, macrolides, aminoglycosides, lincosamides and others.
How to use Eclipse 3G test kit:
1.Add 100 µl of milk samples into micro wells.
2.Incubate 2,5 hours at 65°C.
3.Wash the wells
4.Visual reading
* 96 test in box
* Compatible with Milkotester ET- W24 thermostatic device
* Detection of β-lactam, tetracycline, sulfamides and other inhibitors
* Easy to use – one step assay.
* Visual reading
* Results in about 2,5h
Limits of detection:
| Antibiotics | Limits of detection(µg/l) |
| β-LACTAMS |
| Penicillin-G | 2 |
| Ampicillin | 3 |
| Amoxicillin | 3 |
| Oxacillin | 10 |
| TETRACYCLINES |
|
| Doxycycline | 10 0 |
| Oxytetracycline | 50 |
| Tetracycline | 100 |
| SULFONAMIDES |
| Sulfadiazine | 100 |
| Sulfametazine | 150 |
| Sulfamethoxypyridazine | 100 |
| Sulfathiazole | 50 |
| MACROLIDES |
| Erythromycin | 200 |
| Tylosin | 40 |
| AMINOGLYCOSIDES |
| Streptomycin | 2.500 |
| Gentamycin | >1.000 |
| Neomycin | >1.000 |
| Espectinomicine | >2.500 |
| LINCOSAMIDES |
| Lincomycin >15 | >15 |
| OTHERS |
| Chloramphenicol | 5.000
|
