Twinsensor test kit 96 tests
(β-lactam and tetracycline)
Twinsensor is a competitive test involving two receptors in one single operation. The test requires the use of two components. The first component is a microwell containing predetermined amounts of both receptors and antibodies linked to gold particles. The second is a dipstick made up of a set of membranes with specific capture lines.
Twinsensor tests are used with dry incubator Milkotester ET- W24. When the thermostatic device has reached the set temperature 40°, then it’s ready for test.
Add 200 µl of milk samples into micro wells and mix.
Incubate 3 min at 40°C.
Dip one Dipstick into each micro well. Continue incubating for 3 min at 40°C.
Read the colour intensities.
Limits of detection:
| Antibiotics | Limits of detection ppb (µg/l) |
| β-lactam antibiotics | Penicillin-G | 2 - 3 |
| Ampicillin | 3 – 5 |
| Amoxicillin | 3 – 5 |
| Oxacillin | 12 - 18 |
| Cloxacillin | 6 – 8 |
| Dicloxacillin | 6 – 8 |
| Nafcillin | 30 – 50 |
| Cefalosporins | Cefacetrile | 30 - 40 |
| Cefalexin | >750 |
| Cefalonium | 3 – 5 |
| Cefazolin | 18 – 22 |
| Cefoperazone | 3 – 4 |
| Cefquinome | 20 - 30 |
| Ceftiofur | 10 – 15 |
| Cephapirin | 6 – 8 |
| Tetracyclines | Chlortetracycline | 30 – 40 |
| Doxycycline | 10 – 15 |
| Oxytetracycline | 50 – 60 |
| Tetracycline | 80 – 100 |
